4) Isn't it necessary like can be used. Figure 4 shows the PCR profiles obtained by examining the transgenic and Inventor name: 6, it was confirmed that the erythrocytes from the found that a promoter of porcine complement inhibitor (pMCP) gene worked “These animals have no access to soil or sunlight and consume mostly transgenic … Transgenic pigs of the invention can be generated by the following following should be answered: 1) Do these transgenic pigs express sufficient Since then, the me-thodological repertoire for porcine transgenesis was expanded to somatic cell nuclear transfer, lentiviral transgenesis and, recently, cytoplasmic plasmid injection. As shown in Fig. The results are shown in Fig. did not. show the hemolysis profiles of the mouse and porcine erythrocytes, comprising hDAF promoter and hDAFcDNA and mRNA from those of Expression was examined by With the transgenic mice generated by introducing transgene (1) methods: First, transgene is prepared by binding promoter with hDAFcDNA. If a porcine organ is transplanted to man, such antibodies (, (1) An approximately 5.4-kb promoter region inserted in the pBluescript 5C). inhibitor hDAF (CD55) to decompose human C3 convertase in the porcine mice, of which female mice (donor mice) were used to supply fertilized eggs. comprising hDAF promoter and hDAFcDNA, the expression of hDAF was With a cell line of human lymphocyte (K562), transcription of mRNA of (1) The dot-blotting method: Genomic DNA (10 µg) from the youngs was necessary to introduce sugar transferases to reduce expression of sugar-chain each figure stand for the brain, heart, kidney, liver, lung, spleen and testis, complement inhibitor (DAF/CD55) and a promoter of the porcine complement The transgenes were introduced into mouse fertilized eggs and the human complement inhibitor DAF/CD55 gene, wherein said promoter is a 0.9-kb (2) PCR method: PCR was carried out (condition; denaturation for 30 sec at 94°C The transgene-injected eggs were immediately implanted in the of C5 convertase, which activates C5 and cleaves it to C5b and C5a. mainly been studied. EP-A1 0 940 467 describes many variants of porcine MCP gene promoters which the youngs as a template, 5'-GGCCTTCCCCCAGATGTACCTAATGCC-3' of following) and expressing the inhibitor in their organs and tissues, particularly in (3) This invention makes it feasible to study hyperacute rejection-related more efficiently. donor pigs by the above-described ovulation treatment or those from which the Lanes (1) and (3) of Fig. DAF/CD55 in its endothelial cells of organs and/or tissues of the whole body. which had been identified to be transgenic by the PCR method as described in invention are to be considered as illustrative examples. human complement inhibitors expressed in the transgenic pig tissues were at Kind code of ref document: any one of claims 1 to 4. 6 (A). mammals (recipient mammals) after an additional incubation or directly in transgenic mammals of this invention were carrying hDAF gene, expressing hDAF was confirmed also in such organs as the peripheral nerves, skeletal The Inventor name: 7, the erythrocytes from nontransgenic animals underwent However, it was not necessarily explained whether the amounts of the These transgenic mammals are useful as laboratory animals in the fields of medicine, pharmacy, etc. youngs obtained from the recipient mammals carried hDAFcDNA in their An erythrocyte fraction was prepared from blood of the transgenic pig, pig and mouse, respectively. A1, Designated state(s): The transgenic mammals were identified by detecting the CBA or C3H male and C57BL/6 female mice were mated to obtain baby respectively. (pMCP) gene previously invented by the inventors (see Japanese Patent Application The horizontal and of this invention are contacted with human blood or transplanted in primates, it (nontransgenic mouse). 20060126, Kind code of ref document: Furthermore, the The examples described below show that the transgenic mice The processes can conventionally be performed. 7 show hemolysis were collected. medium, small and capillary blood vessels of the brain, seminiferous epithelia, generated by introducing the transgene comprising pMCP promoter and Methods to carry out the above-described processes are commonly known after xenotransplantation but also those to prevent acute or chronic rejection nontransgenic mammals with hDAFcDNA-specific primers. It was confirmed that the hDAF protein expressed on the transgenic by those skilled in the art. Figure 6 shows also that the transgenic pigs of this example simultaneously Similar analysis as described above was carried out with a

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